Assessment of Anti-oxidant Activity of Aerial Parts of Mangrove Plant, Derris trifoliata ( Leguminosae)



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Submitted Jan 12, 2021
Published Sep 27, 2021
10.24018/ejpharma.2021.1.1.4

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The current study was conducted to verify the traditional medicinal use and to carry out the in-vitro antioxidant activity of various solvent extracts of Derris trifoliata (aerial part). The percentage yield of ethanol, ethyl acetate and n-hexane extracts were found 2.5% w/w. Freshly prepared extracts were subjected to preliminary phytochemical screening. All extracts revealed the presence of several important phytochemicals which might be responsible for its medicinal properties. In vitro Electron transfer (ET) reaction-based assays of ethanol, ethyl acetate and n-hexane extracts have been investigated using various model systems viz., DPPH, total phenolic, tannin and flavonoid content, ferric ion reducing antioxidant power (FRAP) and reducing power assay. Hydrogen atom transfer (HAT) reaction-based assays have been conducted using Nitric Oxide (NO) scavenging and hydrogen peroxide scavenging activity assay methods. Ethanol extract was found to possess highest DPPH (IC50=16.824 µg/ml), total phenolic content (44.51 GAE/g of dried plant extract), reducing power assay (0.387±0.0006), FRAF assay (IC50=133.51 µg/ml), hydrogen peroxide scavenging (IC50=144.888 µg/ml) and nitric oxide scavenging activities (IC50=152.655 µg/ml). Whereas ethyl acetate extract was found to possess the highest total tannin content (42.56 GAE/g of dried plant extract) and total flavonoid content (78.08 QE/g of dried plant extract). In vitro antioxidant study was also performed in terms of chelation power on ferrous ions. The highest chelation power was found for ethyl acetate extract (IC50=62.489 µg/ml). The above study suggests that Derris trifoliata may be a vital source of nutraceuticals.

Keywords: Derris trifoliata, Quantitative anti-oxidant activity, Scavenging and assay, Total phenol tannin and flavonoid content

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References

  1. Gani, Medicinal Plants of Bangladesh, 2nd ed. Bangladesh: The Asiatic Society of Bangladesh; pp. 45-47, 2003.
     Google Scholar
  2. J. D. Witt, S. L. Warren, T. G. Ranney and J. R. Baker, “Biorational and Conventional Plant Protectants Reduce Feeding by Adult Japanese Beetles,” J. Environ. Hortic, vol. 17, pp. 203–206, 1999.
     Google Scholar
  3. K.R. Kirtikar and B.D. Basu, “Indian Medicinal Plants,” Dehradun, India: International Book Distributors; 1987.
     Google Scholar
  4. A. Bhattacharyya and C.R. Babu, “Purification and Biochemical Characterization of a Serine Proteinase Inhibitor from D. trifoliata Lour. Seeds: Insight into Structural and Antimalarial Features,” Phytochemistry, vol. 70, no. 6, pp. 703–712, 2009.
     Google Scholar
  5. M.R. Khan, A.D. Omoloso and Y. Barewai, “Antimicrobial Activity of the D. Elliptica, D. Indica and D. Trifoliata Extractives,” Fitoterapia, vol. 77, pp. 327–330, 2006.
     Google Scholar
  6. S. Tewtrakul, S. Cheenpracha and C. Karalai, “Nitric Oxide Inhibitory Principles from D. trifoliata Stems,” Phytomedicine, vol. 16, pp. 568–572, 2009.
     Google Scholar
  7. A. Yenesew, H. Twinomuhwezi, J.M. Kabaru, H.M Akala, B.T. Kiremire, M. Heydenreich, M.G. Peter, F.L. Eyase, N.C. Waters and D.D. Walsh, “Antiplasmodial and larvicidal flavonoids from Derris trifoliata.” Bull Chem Soc Ethiop vol. 23, pp. 409–414, 2009.
     Google Scholar
  8. C.M.R. Gomes, O.R. Gottlieb, G.B.M. Bettolo, F. Dellemonache and R.M. Polhill, “Systematic Significance of Flavonoids in D. and Lonchocarpus,” Biochemical Systematics and Ecology, vol. 9, pp. 129–147, 1981.
     Google Scholar
  9. G.E. Trease and W.C. Evans, “Pharmacognosy,” 13th ed. London: Bailliere Tindall; pp. 176-180, 1989.
     Google Scholar
  10. A. Prakash, “Antioxidant Activity, Analytical progress report of Medallion Laboratories,” vol. 19, no. 2, pp. 1-2, 2001.
     Google Scholar
  11. G. Cioffi, M. D’Auria, A. Braca, J. Mendez, A. Castillo, I. Morelli, F. De Simone and N. De Tommasi, “Antioxidant and Free Radical ScavengingActivity of Constituents of the Leaves of Tachigaliapaniculata,” Journal of Natural Products, vol. 65, pp. 1526-1529, 2002.
     Google Scholar
  12. K. Wolfe, X. Wu and R.H. Liu, “Antioxidant activity of apple peels,” Journal of Agricultural and Food Chemistry, vol. 51, pp. 609−614, 2003.
     Google Scholar
  13. E.L.C. Amorim, J.E. Nascimento, J.M. Monteiro, S. Peixoto, T.A.S. Araujo and U.A.P. Albuquerque, “A simple and accurate procedure for the determination of tannin and flavonoid levels and some applications in ethnobotany and ethnopharmacology,” Functional Ecosystem and Communities, vol. 2, pp. 88-9, 2008.
     Google Scholar
  14. S. Monic, R.B. Yerram and B. Jagadeesh, “Phytochemical screening and in- vitro antioxidant activity of aqueous and hydroalcoholic extract of Bacopa monnieri Linn,” IJPSR, vol. 3, no. 9, pp. 3418-3424, 2012.
     Google Scholar
  15. M. Oyaizu, “Studies on product of browning reaction prepared from glucosamine,” Japan Journal of Nutrition, vol. 44, pp. 307-315, 1986.
     Google Scholar
  16. G. Sudha, M.S Priya, R.I Shree and S. Vadivukkarasi, “In vitro free radical scavenging activity of raw pepino fruit (solanum muricatum aito),” International Journal of Current Pharmaceutical Research, vol. 3, no. 2, pp. 137-140, 2011.
     Google Scholar
  17. F.N. Seyed, M.N. Seyed, A.E. Mohammad and A. Hossein, “The antioxidant activity of wild medlar (Mespilusgermanica L.) fruit, stem bark and leaf,” African Journal of Biotechnology, vol. 10, no. 2, pp. 283-289, 2011.
     Google Scholar
  18. R.J. Ruch, S.J. Cheng and J.E. Klaunig, “Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea,” Carcinogenesis, vol. 10, pp.1003, 1989.
     Google Scholar
  19. L.C. Green, D.A. Wagner, J. Glogowiski, P.L. Skipper, J.S. Wishnok and S.R. Tannenbaum, “Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids,” Analytica Biochem, 126: 131-138, 1982.
     Google Scholar
  20. A. Sharma, S. Bhardwaj, A. Mann, A. Jain and M. Kharya, “Screening Methods of Antioxidant Activity: An Overview,” Pharmacognosy reviews, vol. 2, pp. 232-238, 2007.
     Google Scholar
  21. I.E.F. Benzie and J.J Strain, “The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay,” Analytica Biochem, vol. 239, no. 1, pp. 70-76, 1996.
     Google Scholar
  22. J.F. Haro-Vicente, C. Martinez-Gracia and G. Ros, “Optimization of in vitro measurement of available iron from different fortificants in citric fruit juices,” Food Chemistry, vol. 98, no.1, pp. 639-648, 2006.
     Google Scholar
  23. P.D. Duh, Y.Y. Tu and G.C. Yen, “Antioxidant activity of water extract of Harug Jyur (Chrysanthemum morifolium Ramat),” Lebensmittel - Wissenchaft und Technologie, vol. 32, no.5, pp. 269–277, 1999.
     Google Scholar
  24. M.H. Gordon, “The mechanism of antioxidant action in vitro,” In: BJF Hudson (Ed), Food antioxidants, Elsevier Applied Science, London. pp 1-18, 1990.
     Google Scholar
  25. J.R. Soares, T.C.P. Dins, A.P. Cunha and L.M. Ameida, “Antioxidant activity of some extracts of Thymus zygis,” Free Radical Research, vol. 26, pp. 469–478, 1997.
     Google Scholar
  26. L.W. Chang, W.J. Yen, S.C. Huang and P.D. Duh, “Antioxidant activity of sesame coat,” Food Chemistry, vol. 78, pp.347–354, 2002.
     Google Scholar
  27. P.D. Duh, Y.Y. Tu and G.C. Yen, “Antioxidant activity of water extract of Harug Jyur (Chrysanthemum morifolium Ramat),” Lebensmittel- Wissenchaft und Technologie, vol. 32, no.5, pp. 269–277, 1999.
     Google Scholar
  28. I. Gülçin, M.T. Uguz, M. Oktay, S. Beydemir and Ö. I. Küfrevioglu, “Antioxidant and antimicrobial activities of Teucrium polium L.” Journal of Food Technology, vol.1, pp. 9-17, 2003.
     Google Scholar
  29. T. Hatano, R. Edamatsu, M. Hiramatsu, A. Mori and Y. Fujita, “Effects of the interaction of tannins with co-existing substances. VI: effects of tannins and related polyphenols on superoxide anion radical and on 1,1-diphenyl-2-picrylhydrazyl radical,” Chemical and Pharmaceutical Bulletin, vol. 37, pp. 2016–2021, 1989.
     Google Scholar
  30. N.P. Das and T.A. Pereira, “Effects of flavonoids on thermal autoxidation of palm oil: structure–activity relationship,” Journal of the American oil chemists’ society, vol. 67, pp. 255–258, 1990.
     Google Scholar
  31. N.S.C. De Gaulejac, Y. Glories and N. Vivas, “Free radical scavenging effect of anthocyanins in red wines,” Food Resource International, vol. 32, pp. 327–333, 1999.
     Google Scholar
  32. N. Tamilselvi, P. Krishnamoorthy, R. Dhamotharan, P. Arumugam and E. Sagadev, “Analysis of total phenols, total tannins and screening of phytocomponents in Indigofera aspalathoides(Shivanar Vembu) Vahl EX DC,” Journal of chemical and pharmaceutical research, vol. 4, no. 6, pp. 3259-3262, 2012.
     Google Scholar
  33. V. Koleckar, K. Kubikova, Z. Rehakova, K. Kuca, D. Jun, L. Jahodar and L. Opletal, “Condensed and hydrolysable tannins as antioxidants influencing the health,” Mini Review in Medicinal Chemistry, vol. 8, pp. 436-447, 2008.
     Google Scholar
  34. V. Amico, R. Chillemi, S. Mangiafico and C. Spatafora, “Tringali, Polyphenol-enriched fractions from Sicilian grape pomace: HPLC-DAD analysis and antioxidant activity,” Bioresource Technology, vol. 99, pp. 5960–5966, 2008.
     Google Scholar
  35. Z. Maksimović, D. Malenčić and N. Kovačević, “Polyphenol contents and antioxidant activity of Maydis stigma extracts,” Bioresource Technology, vol. 96, pp. 873–877, 2005.
     Google Scholar
  36. P. Ranju, G. Kundlik, S. Nidhi, M.H. Mohammed and N. Thirumoorthy, “Antioxidant and free radical scavenging activity of ethanolic extract of Morinda citrifolia,” Annals of Biological Research, vol. 2, no. 1, pp. 127-131, 2011.
     Google Scholar
  37. V. Nagavani, Y. Madhavi, R.D. Bhaskar, R.P. Koteswara and R.T. Raghava, “Free radical scavenging activity and qualitative analysis of polyphenols by RP-HPLC in the flowers of Couroupita guianensis, Electronic Journal of Environmental,” Agricultural and Food Chemistry, vol. 9, no. 9, pp. 1471-1484, 2010.
     Google Scholar
  38. K. Nagarajan, A. Mazumder and L.K. Ghosh, “In-vitro antioxidant activity of alcoholic extracts of Wrightia Tomentosa,” Pharmacologyonline, vol. 1, pp. 196-203, 2008.
     Google Scholar
  39. R.L. Prior and G.H. Cao, “In-vivo total antioxidant capacity: comparison of different analytical methods,” Free Radical Biology and Medicine, vol. 27, pp. 1173-1181, 1999.
     Google Scholar
  40. M.J. Jung, S.I. Heo and M.H. Wang, “Free radical scavenging and total phenolic contents from methanolic extracts of Ulmus davidiana,” Food Chemistry, vol.108, pp. 482-487, 2008.
     Google Scholar
  41. M.J. Miller, H. Sadowska-Krowicka, S. Chotinaruemol, J.L. Kakkis and D.A. Clark, “Amelioration of chronic ileitis by nitric oxide synthase inhibition,” Journal of pharmacology and experimental therapeutics, vol. 264, no. 1, pp. 11-16, 1993.
     Google Scholar
  42. B.S. Tylor, Y.M. Kion, Q.I. Wang, R.A. Sharpio, T.R. Billiar and D.A. Geller, “Nitric oxide down-regulates hepatocyte-inducible nitric oxide synthase gene expression,” Archives of Surgery, vol. 132, pp. 1177–1183, 1997.
     Google Scholar
  43. H. Ischiropoulos, A.B. al-Mehdi and A.B. Fisher, “Reactive species in ischemic rat lung injury: Contribution of peroxynitrite,” American Journal of Physiology, vol. 269, pp. 158–164, 1995.
     Google Scholar
  44. C. Guo, J. Yang, J. Wei, Y. Li, J. Xu and Y. Jiang, “Antioxidant activities of peel, pulp and seed fractions as determined by FRAP assay,” Nutrition Research, vol. 23, pp. 1719-1726, 2003.
     Google Scholar
  45. B. Ercan, and K. Ekrem, “Evaluation of reducing power and radical scavenging activities of water and ethanol extracts from sumac (Rhus coriaria L.),” Food Research International, vol. 44, pp. 2217–2221, 2011.
     Google Scholar
  46. S. Meir, J. Kanner, B. Akiri and S. Philosoph-Hadas, “Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves,” Journal of Agricultural and Food Chemistry, vol. 43, pp. 1813–1819, 1995.
     Google Scholar
  47. Y. Li, C. Guo, J. Yang, J. Wei, J. Xu and S. Cheng, “Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract,” Food Chemistry, vol. 96, pp. 254–260, 2006.
     Google Scholar
  48. I.E.F. Benzie and J.J Strain, “The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay,” Analytica Biochem, vol. 239, no. 1, pp. 70-76, 1996.
     Google Scholar
  49. Y. Yamaji, Y. Nakazato, N. Oshima, M. Hayashi and T. Saruta, “Oxidative stress induced by iron released from transferring in low pH peritoneal dialysis solution,” Nephrology Dialysis Transplantation, vol. 19, no. 10, pp. 2592–2597,2004.
     Google Scholar
  50. E.R. Stadtman, “Protein oxidation and aging,” Science, vol. 257, no. 5074, pp. 1220–1224, 1992.
     Google Scholar
  51. L.W. Chang, W.J. Yen, S.C. Huang and P.D. Duh, “Antioxidant activity of sesame coat,” Food Chemistry, vol. 78, pp.347–354, 2002.
     Google Scholar